Binary vector pcambia-1300
WebAug 14, 2015 · Grains of an insect resistant transgenic Bt rice (Oryza sativa L. Japonica cv Ariete), transformed by Agrobacterium tumefaciens with the binary vector pCAMBIA 1300 containing the synthetic Cry1B gene from Bacillus thuringiensis, and its corresponding wild-type (WT) were used in this study . The CRM of the GM maize MON863 9.85 % (ERM … WebpCAMBIA1300 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available …
Binary vector pcambia-1300
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WebEnter the email address you signed up with and we'll email you a reset link. WebNov 15, 2024 · The pCambia-1300 binary vector was used as a backbone. A pCambia–BSCTV vector was used as template to amplify pV86 or pC86 trans-activated promoters using primers pV86-GUS-R1-F/R or pC86 …
WebThe pBIN19 vector was developed in the 1980s and is one of the first and most widely used binary vectors. The pGreen vector, which was developed in 2000, is a newer version of … WebThe cDNA was cloned into a binary vector (pCAMBIA 1300) and transformed into indica rice var Pusa Basmati-1. Southern hybridization analysis of transgenic rice plants revealed stable integration of the Sod1 transgene in the rice genome. The mRNA transcript of Sod1 was detected by Northern hybridisation in the transgenic rice plants.
WebJul 24, 2013 · A twin T-DNA system is a convenient strategy for creating selectable marker-free transgenic plants. The standard transformation plasmid, pCAMBIA 1300, was modified into a binary vector consisting of two separate T-DNAs, one of which contained the hygromycin phosphotransferase ( hpt) marker gene. WebA twin T-DNA system is a convenient strategy for creating selectable marker-free transgenic plants. The standard transformation plasmid, pCAMBIA 1300, was modified into a binary vector consisting of two separate T-DNAs, one of which contained the hygromycin phosphotransferase (hpt) marker gene.
WebJun 19, 2007 · Biological Sciences Two type III effector genes of Xanthomonas oryzae pv. oryzae control the induction of the host genes OsTFIIA γ 1 and OsTFX1 during bacterial blight of rice Akiko Sugio, Bing Yang, Tong Zhu, and Frank F. White Authors Info & …
WebJun 9, 2024 · In the first step, an EcoRI/KpnI fragment of ~ 2.4 kb was excised from the plasmid pKSMYMIV-A and cloned into the EcoRI/KpnI sites of binary vector pCAMBIA-1300 (Cambia) to produce the recombinant plasmid pCAM-MYMIV-AM. list of festivals in alberta wikipediaWebThe vector pCAMBIA- 1300: SniOLP was digested with Eco RI enzyme and made blunt ended by end filling. This blunt-ended vector was used for cloning the blunt-ended Rs-AFP2 expression cassette. imagine key blockout calendarWebpCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system. The pCambia1300 vector offers: High copy number in E.coli for high DNA yields imagine john lennon by c fernandoWebJun 1, 2024 · The new plant RNAi binary vector, named pCAMBIA2301-GW-RNAi, has two inverted repeated Gateway cassettes driven by the cauliflower mosaic virus 35S (CaMV … imaginek12 educationWebBinary vector pCAMBIA-1301 Taxonomy ID: 118389 (for references in articles please use NCBI:txid118389) current name. Binary vector pCAMBIA-1301. NCBI BLAST name: other sequences Rank: species Genetic code: Translation table 1 (Standard) Plastid genetic code: Translation table 11 (Bacterial, Archaeal and Plant Plastid) list of festivals in 2023 in indiaWebSep 8, 2014 · In this study, a novel binary T-vector was constructed by integrating two AhdI restriction sites into the backbone vector pCAMBIA 1300. The T-vector also contains a … list of fertilizer stocks in indiaWebSep 8, 2014 · In this study, a novel binary T-vector was constructed by integrating two AhdI restriction sites into the backbone vector pCAMBIA 1300. The T-vector also contains a … imagine john lennon impact on society